Paper microfluidics is a rapidly growing subfield of microfluidics that makes use of paper-like porous materials to create analytical devices. Advantages of the use of porous materials include capillary flow, removing the need for equipment for pumping fluids, and lower material costs compared to traditional microfluidics-based devices composed of silicon or glass. The focus of this seminar will be on the development of paper-based devices to automatically perform multistep sample processing for high-sensitivity detection in a format that is appropriate for use in low-resource settings. The discussion will include novel tools for controlling fluid flow in paper microfluidic devices and examples of multistep sample processing in the context of human disease diagnosis.